OOActive is Artificial Oocyte Activation (AOA) with calcium ionophore-an advanced IVF lab technique used after ICSI for selected patients who’ve faced failed or very low fertilisation. Sometimes, even when the sperm is injected correctly during ICSI, the egg doesn’t “switch on” (activate) the way it should. OOActive supports that activation step by using a calcium ionophore solution, which helps increase calcium inside the egg-similar to the natural signal the sperm usually triggers.
OOActive is considered only when your case strongly suggests an egg activation issue after ICSI. Professional guidance also recommends restricting AOA to very specific situations (not for everyone).
If fertilisation is failed or consistently low, AOA with calcium ionophore may help trigger activation so fertilisation can begin.
International best-practice guidance suggests considering AOA when previous routine ICSI cycles resulted in 30% or no fertilisation (after proper review of egg/sperm/ICSI quality).
Some eggs don’t release the internal calcium signal needed to start fertilisation. AOA supports this calcium-based activation pathway.
In some male-factor conditions (including rare cases like globozoospermia), the sperm may lack the factor needed to activate the egg, and AOA can be particularly helpful in this subgroup.
If standard checks look normal but fertilisation still fails, OOActive may be discussed as a next-step lab strategy.
OOActive is done immediately after ICSI inside the embryology lab:
OOActive can improve fertilisation rates in selected patients, especially those with prior low/failed fertilisation after ICSI.
Advanced fertility treatments supported by experienced specialists, modern technology, and structured care throughout your treatment journey.
If you’ve had failed fertilisation after ICSI or repeated low fertilisation, speak with the fertility team at India IVF to understand whether OOActive (AOA with calcium ionophore) fits your case.
Understanding your options is the first step toward hope. Here are some of the most common questions our fertility experts answer every day.
